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PUBLICATIONS

Books

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Book Chapters

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1. Loh Q, Lim TS (2016) DNA G-Quadruplex as a Reporter System for Sensor Development Nucleic Acids-From Basic Aspects to Laboratory Tools. InTech

 

2. Ch’ng ACW, Choong YS, Lim TS (2016) Phage Display‐Derived Antibodies: Application of Recombinant Antibodies for Diagnostics Proof and Concepts in Rapid Diagnostic Tests and Technologies. InTech

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3. Ch’ng ACW, Hamidon NHB, Konthur Z, Lim TS (2018) Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection Phage Display. Springer, pp 301-319

 

4. Chan SK, Choong YS, Gan CY, Lim TS (2018) Chemoenzymatic Bioconjugation of Antibodies: Linking Proteins for Biomedical Applications Enzymes in Food Technology. Springer, pp 359-381

 

5. Chan SK, Lim TS (2018) Immune Human Antibody Libraries and Infectious Diseases Recombinant Antibodies for Infectious Diseases. Springer

 

6. Chan SK, Rahumatullah A, Lai JY, Lim TS (2018) Naive Human   Antibody Libraries for Infectious Diseases. Recombinant Antibodies for Infectious Diseases. Springer

 

7. Lim CC, Choong YS, Lim TS (2018) High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires Antibody Engineering. InTech

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8. Ch'ng ACW, Hamidon NHB, Konthur Z, Lim TS. (2018) Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection. In: Methods Mol Biol. Phage Display, Springer (Hust, M. & Lim TS, Eds.) 1701:301-319. ISBN 978-1-4939-7446-7.

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9. Chin CF, Choong YS, Lim TS. (2018) Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T's®) Antibody Phage Display Biopanning. In: Methods Mol Biol. Phage Display, Springer (Hust, M. & Lim TS, Eds.) 1701:285-299. ISBN 978-1-4939-7446-7.      

 

10.  Omar N, Lim TS. (2018) Construction of Naive and Immune Human Fab Phage-Display Library. In: Methods Mol Biol. Phage Display, Springer (Hust, M. & Lim TS, Eds.) 1701:25-44. ISBN 978-1-4939-7446-7.

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11. Chan SK, Choong YS, Gan CY, Lim TS. (2018) Chemoenzymatic Bioconjugation of Antibodies: Linking Proteins for Biomedical Applications. In: Enzymes in Food Technology: Improvements and Innovations, Springer (Kuddus M, Ed.) 359-381. ISBN 978-981-13-1932-7.

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12. Ch'ng ACW, Ahmad A, Konthur Z, Lim TS. (2019) A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning. In: Methods in Mol. Bio. Human Monoclonal Antibodies, Springer (Steinitz M. Ed.) 1904:377-400. ISBN 978-1-4939-8957-7.

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13. Ch'ng ACW, Konthur Z, Lim TS. (2020) Magnetic Bead-Based Semi-Automated Phage Display Panning Strategy For The Directed Evolution of Antibodies. In: Methods in Enzymology. Nanoarmoring of Enzymes with Carbon Nanotubes and Magnetic Nanoparticles. Vol 630, Elsevier (Kumar C.V., Ed.) 159-178. ISBN 978-0-1282-0143-5.

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14. Lai JY, Lim TS. (2022) Antibody Phage Display. In: Encyclopedia of Infection and Immunity. Vol 4, Elsevier (Rezaei N., Ed.) 215-227. ISBN 978-0-323-90303-5.

Peer Reviewed Articles

  1. Ch'ng ACW, Lam P, Alassiri M, Lim TS. Application of phage display for T-cell receptor discovery. Biotechnol Adv. 2022 Jan-Feb;54:107870. doi: 10.1016/j.biotechadv.2021.107870. Epub 2021 Nov 18. PMID: 34801662.

  2. Yeoh SG, Sum JS, Lai JY, W Isa WYH, Lim TS. Potential of Phage Display Antibody Technology for Cardiovascular Disease Immunotherapy. J Cardiovasc Transl Res. 2022;15(2):360-380. doi: 10.1007/s12265-021-10169-x. Epub 2021 Aug 31. PMID: 34467463. 

  3. Choong YS, Lim TS, Liu H, Jiang R, Cai Z, Ge Y. Potential Inhibition of COVID-19 RNA-dependent RNA Polymerase by Hepatitis C Virus Non-nucleoside Inhibitors: An In-silico Perspective. Letters in Drug Design & Discovery, 2021, 18, 1-7. doi: 10.2174/1570180817999201104123750.

  4. Glökler J, Lim TS, Ida J, Frohme M. Isothermal amplifications - a comprehensive review on current methods. Crit Rev Biochem Mol Biol. 2021 Dec;56(6):543-586. doi: 10.1080/10409238.2021.1937927. Epub 2021 Jul 15. PMID: 34263688.

  5. Balachandra D, Rahumatullah A, Lim TS, Mustafa FH, Ahmad H, Anuar NS, Noordin R. A new antigen detection ELISA for the diagnosis of Strongyloides infection. Acta Trop. 2021 May 28:105986. doi: 10.1016/j.actatropica.2021.105986. Epub ahead of print. PMID: 34058161. 

  6. Lim CC, Chan SK, Lim YY, Ishikawa Y, Choong YS, Nagaoka Y, Lim TS. Development and structural characterisation of human scFv targeting MDM2 spliced variant MDM215kDa. Mol Immunol. 2021 Apr 27;135:191-203. doi: 10.1016/j.molimm.2021.04.016. Epub ahead of print. PMID: 33930714.

  7. Arnaout RA, Prak ETL, Schwab N, Rubelt F; Adaptive Immune Receptor Repertoire Community. The Future of Blood Testing Is the Immunome. Front Immunol. 2021 Mar 15;12:626793. doi: 10.3389/fimmu.2021.626793. PMID: 33790897; PMCID: PMC8005722.

  8. Lai JY, Inoue N, Oo CW, Kawasaki H, Lim TS. One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties. RSC Adv., 2021; 11: 1367-1375. doi: 10.1039/D0RA02835E

  9. Rahumatullah A, Balachandra D, Noordin R, Baharudeen Z, Lim YY, Choong YS, Lim TS. Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides. Sci Rep. 2021; 11(1):2502. doi: 10.1038/s41598-021-82125-3.

  10. Mohd Ali MR, Sum JS, Aminuddin Baki NN, Choong YS, Nor Amdan NA, Amran F, Lim TS. Development of monoclonal antibodies against recombinant LipL21 protein of pathogenic Leptospira through phage display technology. Int J Biol Macromol. 2021; 168:289-300. doi: 10.1016/j.ijbiomac.2020.12.062

  11. Rahumatullah A, Ahmad A, Noordin R, Lai JY, Baharudeen Z, Lim TS. Applicability of Brugia malayi immune antibody library for the isolation of a human recombinant monoclonal antibody to Echinococcus granulosus antigen B. Exp Parasitol.;219:108029. doi: 10.1016/j.exppara.2020.108029. 

  12. Ibeji CU, Salleh NAM, Sum JS, Ch'ng ACW, Lim TS, Choong YS. Demystifying the catalytic pathway of Mycobacterium tuberculosis isocitrate lyase. Sci Rep. 2020; 10(1):18925. doi: 10.1038/s41598-020-75799-8. 

  13. Ida J, Kuzuya A, Choong YS, Lim TS. An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection. RSC Advances. 2020; 10: 33040-33051. doi: 10.1039/D0RA05439A

  14. Lai JY, Lim TS. Infectious disease antibodies for biomedical applications: A mini review of immune antibody phage library repertoire. Int J Biol Macromol. 2020; 163:640-648. doi: 10.1016/j.ijbiomac.2020.06.268. 

  15. Lim YY, Lim TS, Choong YS. Human IgG1 Fc pH-dependent optimization from a constant pH molecular dynamics simulation analysis. RSC Adv., 2020 (10): 13066-13075. doi: 10.1039/C9RA10712F

  16. Rahumatullah A, Lim TS, Yunus MH, Noordin R. Development of an Antigen Detection ELISA for Bancroftian Filariasis Using BmSXP-Specific Recombinant Monoclonal Antibody. Am J Trop Med Hyg. 2019 Aug;101(2):436-440. doi: 10.4269/ajtmh.19-0034. PMID: 31162018

  17. Lim YY, Lim TS, Choong YS. Structural approaches for the DNA binding motifs prediction in Bacillus thuringiensis sigma-E transcription factor (σETF). J Mol Model. 2019 Sep 5;25(10):301. doi: 10.1007/s00894-019-4192-3. PMID: 31486892.

  18. Lim CC, Choong YS, Lim TS. Cognizance of Molecular Methods for the Generation of Mutagenic Phage Display Antibody Libraries for Affinity Maturation. Int J Mol Sci. 2019 Apr 15;20(8). pii: E1861. doi: 10.3390/ijms20081861. Review. PubMed PMID: 30991723.

  19. Lim CC, Woo PCY, Lim TS. Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies. Sci Rep. 2019 Apr 15;9(1):6088. doi: 10.1038/s41598-019-42628-6. PubMed PMID: 30988390; PubMed Central PMCID: PMC6465254.

  20. Ch'ng ACW, Chan SK, Ignatius J, Lim TS. Human T-cell receptor V gene segment of alpha and beta families: A revised primer design strategy. Eur J Immunol. 2019 Mar 28. 49(8): 1186-1199. doi: 10.1002/eji.201747328. PubMed PMID: 30919413.

  21. Ida J, Chan SK, Glökler J, Lim YY, Choong YS, Lim TS. G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing. Molecules. 2019 Mar 19;24(6). pii: E1079. doi: 10.3390/molecules24061079. Review. PubMed PMID: 30893817.

  22. Lee YV, Choi SB, Wahab HA, Lim TS, Choong YS. Applications of Ensemble Docking in Potential Inhibitor Screening for Mycobacterium tuberculosis Isocitrate Lyase Using a Local Plant Database. J Chem Inf Model. 2019 Mar 14. doi: 10.1021/acs.jcim.8b00963. PubMed PMID: 30840452.

  23. Chan SK, Lim TS. Bioengineering of microbial transglutaminase for biomedical applications. Appl Microbiol Biotechnol. 2019 Apr;103(7):2973-2984. doi:10.1007/s00253-019-09669-3. Epub 2019 Feb 25. Review. PubMed PMID: 30805670.

  24. Lai JY, Klatt S, Lim TS. Potential application of Leishmania tarentolae as an alternative platform for antibody expression. Crit Rev Biotechnol. 2019 May;39(3):380-394. doi: 10.1080/07388551.2019.1566206. Epub 2019 Feb 5. PubMed PMID: 30720351.

  25. Soong JX, Chan SK, Lim TS, Choong YS. Optimisation of human V(H) domain antibodies specific to Mycobacterium tuberculosis heat shock protein (HSP16.3). J Comput Aided Mol Des. 2019 Mar;33(3):375-385. doi: 10.1007/s10822-019-00186-z. Epub 2019 Jan 28. PubMed PMID: 30689080.

  26. Lai JY, Loh Q, Choong YS, Lim TS. Cassette hybridization for vector assembly application in antibody chain shuffling. Biotechniques. 2018 Nov;65(5):269-274. doi: 10.2144/btn-2018-0031. PubMed PMID: 30394125.

  27. Chan SK, Kuzuya A, Choong YS, Lim TS. DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping. SLAS Discov. 2019 Jan;24(1):68-76. doi: 10.1177/2472555218791743. Epub 2018 Jul 31. PubMed PMID: 30063871.

  28. Ng WK, Lim TS, Lai NS. Human neonatal Fc receptor as a new potential antibody binding protein for antibody immobilization. Biotechnol Appl Biochem. 2018 Jul;65(4):547-553. doi: 10.1002/bab.1636. Epub 2018 Jan 16. PubMed PMID: 29280199.

  29. Leong SW, Lim TS, Ismail A, Choong YS. Integration of molecular dynamics simulation and hotspot residues grafting for de novo scFv design against Salmonella Typhi TolC protein. J Mol Recognit. 2018 May;31(5):e2695. doi: 10.1002/jmr.2695. Epub 2017 Dec 12. PubMed PMID: 29230887.

  30. Rahumatullah A, Abdul Karim IZ, Noordin R, Lim TS. Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen. Int J Mol Sci. 2017 Nov 22;18(11). pii: E2376. doi: 10.3390/ijms18112376. PubMed PMID: 29165352; PubMed Central PMCID: PMC5713345.

  31. Hamidon NH, Suraiya S, Sarmiento ME, Acosta A, Norazmi MN, Lim TS. Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections. Appl Biochem Biotechnol. 2018 Mar;184(3):852-868. doi: 10.1007/s12010-017-2582-5. Epub 2017 Sep 7. PubMed PMID: 28884285.

  32. Omar N, Hamidon NH, Yunus MH, Noordin R, Choong YS, Lim TS. Generation and selection of naïve Fab library for parasitic antigen: Anti-BmSXP antibodies for lymphatic filariasis. Biotechnol Appl Biochem. 2018 May;65(3):346-354. doi:10.1002/bab.1591. Epub 2017 Sep 14. PubMed PMID: 28833498.

  33. Chan SK, Choong YS, Perera D, Lim TS. Dengue Serotyping With A Label-free DNA Sensor. Anal Methods. 2018;10:214-222. doi: 10.1039/C7AY02131C.

  34. Khor BY, Lim TS, Noordin R, Choong YS. The design of target specific antibodies (scFv) by applying de novo workflow: Case study on BmR1 antigen from Brugia malayi. J Mol Graph Model. 2017 Sep;76:543-550. doi: 10.1016/j.jmgm.2017.07.004. Epub 2017 Jul 19. PubMed PMID: 28811153.

  35. Chin CF, Lai JY, Choong YS, Anthony AA, Ismail A, Lim TS. Delineation of B-cell Epitopes of Salmonella enterica serovar Typhi Hemolysin E: Potential antibody therapeutic target. Sci Rep. 2017 May 19;7(1):2176. doi: 10.1038/s41598-017-01987-8. PubMed PMID: 28526816; PubMed Central PMCID: PMC5438399.

  36. Fischbach J, Loh Q, Bier FF, Lim TS, Frohme M, Glökler J. Alizarin Red S for nline Pyrophosphate Detection Identified by a Rapid Screening Method. Sci Rep. 2017 Mar 24;7:45085. doi: 10.1038/srep45085. PubMed PMID: 28338022; PubMed Central PMCID: PMC5364467.

  37. Chan SK, Lim TS. Immune Human Antibody Libraries for Infectious Diseases. Adv Exp Med Biol. 2017;1053:61-78. 

  38. Chan SK, Rahumatullah A, Lai JY, Lim TS. Naïve Human Antibody Libraries for Infectious Diseases. Adv Exp Med Biol. 2017;1053:35-59.

  39. Park YH, Hamidon F, Rajangan C, Soh KP, Gan CY, Lim TS, Abdullah WN, Liong MT. Application of Probiotics for the Production of Safe and High-quality Poultry Meat. Korean J Food Sci Anim Resour. 2016 Oct 31;36(5):567-576. PubMed PMID: 27857531; PubMed Central PMCID: PMC5112418.

  40. Lim TS. Editorial: Antibody Phage Display: The Evolution of Antibody Therapeutics. Curr Pharm Des. 2016;22(43):6477-6479. doi: 10.2174/1381612822999161019110228. PubMed PMID: 27781936.

  41. Lim TS, Chan SK. Immune Antibody Libraries: Manipulating The Diverse Immune Repertoire for Antibody Discovery. Curr Pharm Des. 2016;22(43):6480-6489. doi: 10.2174/1381612822666160923111924. Review. PubMed PMID: 27669969.

  42. Siow HL, Lim TS, Gan CY. Development of a workflow for screening and identification of α-amylase inhibitory peptides from food source using an integrated Bioinformatics-phage display approach: Case study - Cumin seed. Food Chem. 2017 Jan 1;214:67-76. doi: 10.1016/j.foodchem.2016.07.069. Epub 2016 Jul 11. PubMed PMID: 27507449.

  43. Ng WK, Lim TS, Lai NS. Expression of soluble human Neonatal Fc-receptor (FcRn) in Escherichia coli through modification of growth environment. Protein Expr Purif. 2016 Nov;127:73-80. doi: 10.1016/j.pep.2016.07.004. Epub 2016 Jul 10. PubMed PMID: 27412717.

  44. Ngoh YY, Lim TS, Gan CY. Screening and identification of five peptides from pinto bean with inhibitory activities against α-amylase using phage display technique. Enzyme Microb Technol. 2016 Jul ;89:76-84. doi:  10.1016/j.enzmictec.2016.04.001. Epub 2016 Apr 4. PubMed PMID: 27233130.

  45. Lim BN, Chin CF, Choong YS, Ismail A, Lim TS. Generation of a naïve human single chain variable fragment (scFv) library for the identification of monoclonal scFv against Salmonella Typhi Hemolysin E antigen. Toxicon. 2016 Jul;117:94-101. doi: 10.1016/j.toxicon.2016.04.032. Epub 2016 Apr 14. PubMed PMID: 27090555.

  46. Ismail NF, Lim TS. Site-specific scFv labelling with invertase via Sortase A mechanism as a platform for antibody-antigen detection using the personal glucose meter. Sci Rep. 2016 Jan 19;6:19338. doi: 10.1038/srep19338. PubMed PMID: 26782912; PubMed Central PMCID: PMC4726117.

  47. Chin CF, Ler LW, Choong YS, Ong EB, Ismail A, Tye GJ, Lim TS. Application of streptavidin mass spectrometric immunoassay tips for immunoaffinity based antibody phage display panning. J Microbiol Methods. 2016 Jan;120:6-14. doi: 10.1016/j.mimet.2015.11.007. Epub 2015 Nov 12. PubMed PMID: 26581498.

  48. Hairul Bahara NH, Chin ST, Choong YS, Lim TS. Construction of a Semisynthetic Human VH Single-Domain Antibody Library and Selection of Domain Antibodies against α-Crystalline of Mycobacterium tuberculosis. J Biomol Screen. 2016 Jan;21(1):35-43. doi: 10.1177/1087057115609144. Epub 2015 Sep 30. PubMed PMID: 26423338.

  49. Chan SK, Lim TS. A straw-housed paper-based colorimetric antibody–antigen sensor. Anal Methods. 2016;8:1431-1436. doi: 10.1039/C5AY01828E.

  50. Ismail NF, Chan SK, Tye GJ, Choong YS, Lim TS. Improved Expression of Single-Chain Fragment Variable Antibodies Devoid of Leader Peptides in the Cytoplasm. Current Proteomics. 2015;12(2):117-123. doi: 10.2174/157016461202150903114319.

  51. Khor BY, Tye GJ, Lim TS, Choong YS. General overview on structure prediction of twilight-zone proteins. Theor Biol Med Model. 2015 Sep 4;12:15. doi: 10.1186/s12976-015-0014-1. Review. PubMed PMID: 26338054; PubMed Central PMCID: PMC4559291.

  52. Rahumatullah A, Ahmad A, Noordin R, Lim TS. Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library. Mol Immunol. 2015 Oct;67(2 Pt B):512-23. doi: 10.1016/j.molimm.2015.07.040. Epub 2015 Aug 12. PubMed PMID: 26277276.

  53. Tye GJ, Lew MH, Choong YS, Lim TS, Sarmiento ME, Acosta A, Norazmi MN. Vaccines for TB: Lessons from the Past Translating into Future Potentials. J Immunol Res. 2015;2015:916780. doi: 10.1155/2015/916780. Epub 2015 Jun 3. Review. PubMed PMID: 26146643; PubMed Central PMCID: PMC4469767.

  54. Loh Q, Leong SW, Tye GJ, Choong YS, Lim TS. Improved Fab presentation on phage surface with the use of molecular chaperone coplasmid system. Anal Biochem. 2015 May 15;477:56-61. doi: 10.1016/j.ab.2015.02.026. Epub 2015 Mar 10. PubMed PMID: 25769419.

  55. Ong EB, Ignatius J, Anthony AA, Aziah I, Ismail A, Lim TS. Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen. Microbiol Immunol. 2015 Jan;59(1):43-7. doi: 10.1111/1348-0421.12211. PubMed PMID: 25399538.

  56. Lim BN, Tye GJ, Choong YS, Ong EB, Ismail A, Lim TS. Principles and application of antibody libraries for infectious diseases. Biotechnol Lett. 2014 Dec;36(12):2381-92. doi: 10.1007/s10529-014-1635-x. Epub 2014 Sep 12. Review. PubMed PMID: 25214212.

  57. Chin ST, Ignatius J, Suraiya S, Tye GJ, Sarmiento ME, Acosta A, Norazmi MN, Lim TS. Comparative study of IgA VH 3 gene usage in healthy TST(-) and TST(+) population exposed to tuberculosis: deep sequencing analysis. Immunology. 2015 Feb;144(2):302-11. doi: 10.1111/imm.12372. PubMed PMID: 25158076; PubMed Central PMCID: PMC4298424.

  58. Chin CF, Teh BA, Anthony AA, Aziah I, Ismail A, Ong EB, Lim TS. Overexpression, purification and validation of antigenic Salmonella enterica serovar Typhi proteins identified from LC-MS/MS. Appl Biochem Biotechnol. 2014 Nov;174(5):1897-906. doi: 10.1007/s12010-014-1173-y. Epub 2014 Aug 23. PubMed PMID: 25149461.

  59. Lee SY, Hairul Bahara NH, Choong YS, Lim TS, Tye GJ. DNA fluorescence shift sensor: a rapid method for the detection of DNA hybridization using silver nanoclusters. J Colloid Interface Sci. 2014 Nov 1;433:183-188. doi: 10.1016/j.jcis.2014.07.033. Epub 2014 Jul 31. PubMed PMID: 25129336.

  60. Leong SW, Lim TS, Tye GJ, Ismail A, Aziah I, Choong YS. Assembly and stability of Salmonella enterica ser. Typhi TolC protein in POPE and DMPE. J Biol Phys. 2014 Sep;40(4):387-400. doi: 10.1007/s10867-014-9357-9. Epub 2014 Jul 11. PubMed PMID: 25011632; PubMed Central PMCID: PMC4119184.

  61. Loh Q, Omar N, Glökler J, Lim TS. IQPA: isothermal nucleic acid amplification-based immunoassay using DNAzyme as the reporter system. Anal Biochem. 2014 Oct 15;463:67-9. doi: 10.1016/j.ab.2014.06.012. Epub 2014 Jun 24. PubMed PMID: 24972268.

  62. Khor BY, Tye GJ, Lim TS, Noordin R, Choong YS. The structure and dynamics of BmR1 protein from Brugia malayi: in silico approaches. Int J Mol Sci. 2014 Jun 19;15(6):11082-99. doi: 10.3390/ijms150611082. PubMed PMID: 24950179; PubMed Central PMCID: PMC4100200.

  63. Omar N, Loh Q, Tye GJ, Choong YS, Noordin R, Glökler J, Lim TS. Development of an antigen-DNAzyme based probe for a direct antibody-antigen assay using the intrinsic DNAzyme activity of a daunomycin aptamer. Sensors (Basel). 2013 Dec 27;14(1):346-55. doi: 10.3390/s140100346. PubMed PMID: 24379042; PubMed Central PMCID: PMC3926560.

  64. Choong YS, Tye GJ, Lim TS. Minireview: applied structural bioinformatics in proteomics. Protein J. 2013 Oct;32(7):505-11. doi: 10.1007/s10930-013-9514-1. Review. PubMed PMID: 24096348.

  65. Ong EB, Anthony AA, Ismail A, Ismail A, Lim TS. Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development. Diagn Microbiol Infect Dis. 2013 Sep;77(1):87-9. doi: 10.1016/j.diagmicrobio.2013.05.010. PubMed PMID: 23790417.

  66. Hairul Bahara NH, Tye GJ, Choong YS, Ong EB, Ismail A, Lim TS. Phage display antibodies for diagnostic applications. Biologicals. 2013 Jul;41(4):209-16. doi: 10.1016/j.biologicals.2013.04.001. Epub 2013 May 3. Review. PubMed PMID: 23647952.

  67. Lim BN, Choong YS, Ismail A, Glökler J, Konthur Z, Lim TS. Directed evolution of nucleotide-based libraries using lambda exonuclease. Biotechniques. 2012 Dec;53(6):357-64. doi: 10.2144/000113964. PubMed PMID: 23227986.

  68. Rubelt F, Sievert V, Knaust F, Diener C, Lim TS, Skriner K, Klipp E, Reinhardt R, Lehrach H, Konthur Z. Onset of immune senescence defined by unbiased pyrosequencing of human immunoglobulin mRNA repertoires. PLoS One. 2012;7(11):e49774. doi: 10.1371/journal.pone.0049774. Epub 2012 Nov 30. PubMed PMID: 23226220; PubMed Central PMCID: PMC3511497.

  69. Tommy YB, Lim TS, Noordin R, Saadatnia G, Choong YS. Theoretical investigation on structural, functional and epitope of a 12 kDa excretory-secretory protein from Toxoplasma gondii. BMC Struct Biol. 2012 Nov 27;12:30. doi: 10.1186/1472-6807-12-30. PubMed PMID: 23181504; PubMed Central PMCID: PMC3542155.

  70. Loh Q, Bahara NH, Choong YS, Lim TS. Assembly of highly diverse genes using degenerate oligonucleotides by temperature cascade. Anal Biochem. 2012 Dec 1;431(1):54-6. doi: 10.1016/j.ab.2012.08.025. Epub 2012 Sep 10. PubMed PMID: 22975202.

  71. Yung-Hung RL, Ismail A, Lim TS, Choong YS. A 35 kDa antigenic protein from Shigella flexneri: in silico structural and functional studies. Biochem Biophys Res Commun. 2011 Nov 18;415(2):229-34. doi: 10.1016/j.bbrc.2011.09.116. Epub 2011 Oct 1. PubMed PMID: 21982766.

  72. Choong YS, Lim TS, Chew AL, Aziah I, Ismail A. Structural and functional studies of a 50 kDa antigenic protein from Salmonella enterica serovar Typhi. J Mol Graph Model. 2011 Apr;29(6):834-42. doi: 10.1016/j.jmgm.2011.01.008. Epub 2011 Mar 2. PubMed PMID: 21371926.

  73. Lim TS, Schütze T, Lehrach H, Glökler J, Konthur Z. Diversity visualization by endonuclease: a rapid assay to monitor diverse nucleotide libraries. Anal Biochem. 2011 Apr 1;411(1):16-21. doi: 10.1016/j.ab.2010.12.024. Epub 2010 Dec 23. PubMed PMID: 21185254.

  74. Lim TS, Mollova S, Rubelt F, Sievert V, Dübel S, Lehrach H, Konthur Z. V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes. N Biotechnol. 2010 May 31;27(2):108-17. doi: 10.1016/j.nbt.2010.01.001. Epub 2010 Jan 18. PubMed PMID: 20083243.

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